关键词: 溺水死亡, 甲状腺, 核苷酸, 应激蛋白, 雄性大鼠

Abstract: Objective　To establish a drowning death rat model and analyze the changes of nucleotide and stress protein levels in thyroid at different time after drowning treatment.Methods　Male Wistar rats were divided into a control group and an experimental group. The control group (50 rats) was subjected to painless euthanasia by ether inhalation, while the experimental group (50 rats) was subjected to drowning in a water tank.5 rats were collected at 0, 2, 4, 6, 8, 12, 18, 24, 36 and 72 h after death, respectively. The thyroid glands were removed. 20 mg was weighed and ground respectively, and then centrifuged for 10 minutes using a low-speed refrigerated centrifuge at 3 500 r/min and the supernatant was obtained by enzyme-linked immunoassay to detect the levels of cAMP, cGMP, c-Fos, Hsp-70, Caspase-9, and Bax according to the instructions of the ELISA kit.Results　The levels of cAMP, cGMP, c-Fos, Hsp-70, Caspase-9 and Bax in thyroid tissue were higher than those in control group at different time after drowning death (P<0.05,P<0.01).The levels of cAMP, cGMP, c-Fos, Hsp-70, Caspase-9 and Bax in both the experimental group and the control group increased gradually from 2 h after death.Hsp-70 to 18 h begins to decrease,cAMP, cGMP and c-Fos began to decrease at 24 h, and Caspase-9 and Bax began to decrease at 36 h.Conclusion　The levels of nucleotide and stress protein in tissues showed a certain trend at different time after death.

Key words: drowning death, thyroid gland, nucleotide, stress protein, male rat